Содержание

СПИСОК СОКРАЩЕНИЙ

ГЛАВА

АДФ	–	аденозин-5'-дифосфат
АК	–	аскорбиновая кислота
АКМ	–	активные кислородные метаболиты
цАМФ	–	аденозин-3',5'-циклофосфат
АОА	–	антиоксидантная активность
АРА	–	антирадикальная активность
АТФ	–	аденозин-5'-трифосфат
АФК	–	активные формы кислорода
БТШ	–	белки теплового шока
БХС	–	биохимическая система
цГМФ	–	гуанозин-3',5'-циклофосфат
ДГК	–	дигидрокверцетин
ДК	–	диеновые коньюгаты
ДНК	–	дезоксирибонуклеиновая кислота
МДА	–	малоновый диальдегид
МК	–	мочевая кислота
НАДН	–	никотинамидадениндинуклеотид, восстановленная форма
ПОЛ	–	перекисное окисление липидов
РНК	–	рибонуклеиновая кислота
РФК	–	радикальные формы кислорода
СОХ	–	циклооксигеназа
ТБК	–	тиобарбитуровая кислота
ФМА	–	форбол-12-меристат-13-ацетат
ФС	–	фагоцитсодержащая система
ФТШ	–	фактор теплового шока
ХЛ	–	хемилюминесценция

Кемпфе- рол	Кверце-тин	Кемп-ферол	Кверце-тин
Яблоки	0-7	20-263	Чёрный виноград	0-2	15
Айва	0	63	Белый виноград	0-2	12
Абрикосы	0-2	25-53	Огородная капуста	210-250	50-110
Груши	0-12	3-28	Краснокочанная капуста	0-2	5-6
Кислая вишня	5-17	23-80	Белокочанная капуста	0-2	0-1
Сладкая вишня	0-6	6-24	Цветная капуста	0-2	0-1
Слива	0-2	0-15	Лук-порей	30-200	0-25
Персики	0-2	0-4	Лук-резанец	10	300
Бузина	0	105-237	Лук репчатый (цветной)	0-2	347
Черника	0-6	105-160	Цикорий	46	1-2
Чёрная смородина	0-10	33-68	Французская фасоль	12	39
Белая смородина	0-2	3-28	Стручковая фасоль	0-2	29
Красная смородина	0-2	2-27	Томаты	0-2	7-8
Ежевика	14	33	Морковь	0-1	0-2
Земляника	12	9	Картофель	0-1	0-2
Малина	0-1	29

Число реакционно-способных ОН-групп	Константы скорости ингибирования
	. )	. )	. )
(-)Эпикатехин	2	1,0	4,0	6,8
(+)Катехин	2	2,2	5,0	6,4
Пикногенол	2	1,8	1,75	43
(-)Эпигаллокатехин	3	4,7	4,7	41
(-)Эпикатехина галлат	5	5,8	4,7	43
(-)Эпигаллокатехина галлат	6	7,1	4,8	65
Пропилгаллат	3	3,1	4,2	26
Р-Глюкогаллин	3	4,4	6,3	65
Пентагаллоил-глюкоза	15	71	20	103
Галлодубильная кислота (танин)	25	31	22	-

ГЛАВА II. МАТЕРИАЛЫ И МЕТОДЫ

ГЛАВА III. РЕЗУЛЬТАТЫ И ОБСУЖДЕНИЯ

Катехин, флавон	Тип ингибирования	IС50 (мкМ)
Катехин	неконкурентное	303,95
Эпикатехин	смешанное	20,48
Эпигаллокатехин	смешанное	10,66
Эпикатехина галлат	смешанное	2,86
Эпигаллокатехина галлат	конкурентное	0,76

Антиоксиданты	САРА в БХС	САОА в БХС	САРА в ФС	САОА в ФС
α-токоферол	2,24х10-5	3,41х10-5	2,43х10-4	3,14х10-5
ДГК	2,0х10-7	1,7х10-6	3,3х10-5	5,0х10-6
АК	1,8х10-7	5,2х10-7	1,1х10-5	1,7х10-6

ВЫВОДЫ

ЛИТЕРАТУРА

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Инкубация раствора липида при 37о С и pH 8 в присутствии ингибитора/катализатора ПОЛ
Время инкубации	1 часинкубации			24 часа инкубации
Концентрация	Fe2+	ДГК-Fe2+	ДГК	Fe2+	ДГК-Fe2+	ДГК
5мМ	104±13	124±12	72±9	124±16	91±8	86±8
10мМ	110±17	105±14	116±13	205±17	120±15	80±5
Инкубация раствора липида при 37о С и pH 4 в присутствии ингибитора/катализатора ПОЛ
5мМ	117±14	106±12	40±4	519±37	241±18	49±4
10мМ	120±14	82±8	12±2	745±54	329±24	50±4
Инкубация раствора липида при 37о С и pH 8 в присутствии ингибитора/катализатора ПОЛ и 10 мМ пероксида водорода
5мМ	1098±140	339±27	662±33	1309±152	107±11	68±7
10мМ	1109±78	450±54	169±15	515±42	124±9	79±8
Инкубация раствора липида при 37о С и pH 4 в присутствии ингибитора/катализатора ПОЛ и 10 мМ пероксида водорода
5мМ	521±37	424±70	147±12	454±36	338±31	34±2
10мМ	850±55	478±49	117±11	445±48	472±45	30±2